Add Awesome Method To Get International Quantitative Lysine Acetylomics Knowledge!

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OBSERVE: After the coupling of the first C-terminal amino acid of all peptides to the amino group of the membrane, the amino teams between the spots and also some of the amino groups within the spot areas do not kind bonds with the amino acids. Be sure that all the purification buffers are at four °C and perform the protein purification within the cold room to keep away from protein denaturation. NOTE: This avoids the coupling of amino acids in the further cycles with the membrane as an alternative of the growing peptide chain.
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